Decoding the technology of mass propagation of Di Linh Gia Hac orchids in Lam Dong

With modern In-vitro technology, for the first time, the Center for Science and Technology Application (Department of Science and Technology of Lam Dong Province) has successfully researched the process of mass propagation of Di Linh Gia Hac orchids.

After irradiation, samples of the fake crane orchids were taken to the separation room to be cultured in nutrient solution for a certain time. (Photo: Nguyen Dung/VNA) In Lam Dong – the “capital” of high-tech agriculture of the country, the application of new science and technology in agriculture is increasingly widespread. Especially for the plant breeding industry to preserve rare genetic resources as well as to develop commercial varieties of these plants. Wild Orchid Di Linh is one of such trees. They are not only mass-produced, but also irradiated to create mutations on demand. Di Linh fake crane – a “famous” orchid of orchid players inside and outside Lam Dong province, often sought to buy with many different prices. However with In-vitro technology As modern as it is today, for the first time, the Center for Science and Technology Application (Lam Dong Department of Science and Technology) has successfully studied the process of mass propagation of this plant. Mass breeding The technique of propagation by plant tissue culture (In-vitro) is no longer strange in Lam Dong province in general and Da Lat city in particular. The application of this technology to propagate the species of Orchid Di Linh was first deployed by the Center for Science and Technology Application. The project was completed, completed and accepted by Mr. Phan Quoc Chinh, Deputy Director of Lam Dong Center for Science and Technology Application and his colleagues. To select the original source of quality seed, the people who carried out the project had to collect Gia Hac Di Linh orchids in Da Lat city and Di Linh district, in orchid gardens that met strict standards. Sampled plants must have an average height of 30-40 cm, have 14-16 pairs of leaves, average trunk diameter of about 4mm and be planted in plastic or terracotta pots. Each tree has 4-5 fruits and the physiological ripening rate is not equal. Selected fruits that should be dark green and physiologically ripe, of the specified size, without cracks, were collected for inclusion in the sample. A seedling of the fake crane orchid has had a mutation: the color of the leaves, the stem has turned white or purple. (Photo: Nguyen Dung/VNA) After being cleaned and disinfected, samples of Di Linh imitation crane will be propagated in-vitro according to the following summary procedure: initial culture stage to create buds and clusters of buds; rapid multiplication stage; rooting stage to create a complete plant. These three stages take about 28 weeks, then the seedlings (5cm tall, 4cm long roots, good growth, dark green leaves) are brought to the nursery to plant and take care of. After the next 24 weeks, the plant can be grown with a height of 5-7cm, with 4-5 leaves. According to Mr. Phan Quoc Chinh, plants grown in an In-vitro environment must ensure the conditions of temperature, humidity and light intensity according to certain cycles. In addition to the results of creating a series of fake crane orchids, the project also built a standard process for propagating this plant by the In-vitro method and can be transferred to other units. “During the implementation, we tried to add ripe banana pulp, coconut water to the culture medium. The results showed that shoot formation and shoot growth also increased faster than normal, “- Mr. Chinh said. Very cheap price After finishing breeding process In-vitro, the first 500 orchid seedlings were brought to the nursery at the Don Duong Experimental Station (Don Duong District) and continued to be tested on many different types of media within 3 months. As a result, the rate of plants living outside the nursery reached 90%. Among the types of substrates put into the experiment, coir fiber media helped the plant grow best, the height of the tree reached 5.6cm; The root length is 3.70cm, the survival rate is 95% and the tree has a large stem, dark green leaves, many leaves. A Di Linh Fake Orchid is eligible for sale for 25,000 VND / tree, very cheap compared to the current market. (Photo: Nguyen Dung/VNA) According to Mr. Nguyen Nhu Chuong, Director of Lam Dong Center for Science and Technology Application, after the acceptance of the project, the Center continues to propagate about 3,000 plants to supply domestic and foreign markets at an export price. selling for only about 25,000 VND/seedling. “The method of tissue culture in the laboratory makes it possible to propagate many times larger numbers than natural breeding. Therefore, the price that the Center is selling to the market today is normal,” said Mr. Chuong. From the results of the study, Center for Applied Science and Technology Lam Dong has carried out the development of basic standards for Gia Hac Di Linh orchid seedlings and received by the Sub-Department of Cultivation and Plant Protection. Thereby, the genetic resources of rare forest orchids originating locally are preserved and developed. According to Mr. Ho Anh Dung, Vice Chairman of Da Lat Flower Association, the quality of In-vitro seedlings will ensure higher uniformity and superiority compared to traditional methods, serving the conservation of seed resources. and commercial purposes. The first-line mother plant has been carefully selected, which will help the seedlings to preserve the best characteristics of the mother plant, not only the Di Linh orchid but also precious plants such as Ngoc Linh ginseng, Camellia flower./ .